Enzyme Commission (EC) Number 3.4.24.33 ( BRENDA | IUBMB )
MDL number MFCD02253021
Popular Documents: Bulletin (PDF) | Specification Sheet (PDF)
Properties:
Related Categories
3.4.x.x Peptidases,
3.x.x.x Hydrolases,
Analytical Reagents,
Analytical/Chromatography,
Application Specific Reagents,
Biochemicals and Reagents,
Endoproteinase Asp-N,
Enzymatic and Chemical Protein Cleavage,
Enzyme Class Index,
Enzymes, Inhibitors, and Substrates,
Mass Spectrometry,
Molecular Biology,
Plant Biotechnology,
Plant Proteomics,
Products for Amino Acid Hydrolysis,
Proteases for Cleavage of Proteins,
Protein Digest,
Protein Identification,
Proteolytic Enzymes,
Proteolytic Enzymes and Substrates,
Proteomics,
Reagents for Amino Acid Analysis,
Selective Proteolytic Enzymes
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form
lyophilized powder
packaging
vial of 2 μg
suitability
suitable for protein sequencing
storage temp.
2-8°C
Description:
Application
Endoproteinase Asp-N from Pseudomonas fragi mutant strain has been used for the digestion of specific proteins to prepare peptides and for the analysis of generated peptides by MS (mass spectrometry) method.
General description
Endoproteinase Asp-N is a metallo endoprotease. It is obtained from a mutant strain of Pseudomonas fragi, which hydrolyzes peptide bonds on the N-terminal side of aspartic and cysteic acid residues. Asp-N is used in proteomics for peptide mapping and protein sequence work due to its highly specific cleavage of peptides.
Safety:
Symbol
GHS07, GHS08
Signal word
Danger
Hazard statements
H315-H319-H334-H335
Precautionary statements
P261-P305 + P351 + P338-P342 + P311
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Faceshields, Gloves