Properties:
| RelatedCategories | Analytical/Chromatography,GELifeSciencesGelFiltrationColumns,GelFiltrationChromatography,HPLC,HPLC&FPLCColumnsforBiopolymerSeparations, |
| material | BedSupport(polyamide) |
| | borosilicateglasscolumn |
| | glassfiber(polypropylenereinforced) |
| shelflife | Pleasebeawarethisproductmaybeshipped90daysbeforetheexpirationdate.Formoreinformationonthebatchspecificexpirationdate,pleasecontacttechnicalservice. |
| molwt | molwt10000-600000 Da(globularproteins) |
| packaging | pkgof1 ea |
| mfr.no. | GEHealthcare,28-9893-35 |
| parameter | <1.0mL/minflowrate |
| | 0.3Mpa(42psi) |
| bedvolume | 120 mL |
| columnL×I.D. | 60 cm×16 mm |
| samplevolume | <5 mL |
| matrix | agarosedextrancomposite |
| | dextran-agaroseparticleplatform |
| matrixactivegroup | dextran-agarosephase |
| avg.part.size | 34 μm |
| cleaning | 1-14 |
| workingrange | 3-12 |
| modeofchromatography | Sizeexclusion(SEC) |
Description:
AnalysisNote
ToviewtheCertificateofAnalysisforthisproduct,pleasevisitwww.geLifesciences.com
.
FeaturesandBenefits
• Highresolutionwithshortruntimesandgoodrecovery.
• Highcapacityathighflowrates.
• Separationscanbescaleduptoproductionlevels.
• Prepackedforconvenienceandreproducibility.
• 1/16”fittingsforconvenientconnectionstoAKTAdesignsystems.
Generaldescription
HiLoad®16/600Superdex®200prepgradeareprepackedXKcolumnsdesignedforhigh-resolutionpreparativegelfiltrationchromatography.ThecolumnisanexcellentchoiceforseparatingMAbsfromdimersandcontaminants.
Superdex®prepgrade(pg)isproducedbycovalentbondingofdextrantohighlycross-linkedagarose.Theseparationpropertiesofthesemediaaredeterminedbythedextrancomponent.Steepselectivitycurvesgiveexcellentresolvingpowerforpeptidesandproteinsinthemolecularweightranges,Mr<10000(Superdex®30pg),Mr3000to70000(Superdex®75pg),andMr10000600000(Superdex®200pg).Themediumcombineshighmechanicalstrengthwithhighhydrophilicity,allowinghighflowrates,andminimalnonspecificinteractions.
Recommendedproducts
DiscoverLiChropurreagentsidealforHPLCorLC-MSanalysis
SuitABIlity
Allcommonlyusedbuffers,1MNaOH,0.1MHCl,1Maceticacid,8Murea,6MguanidineHCl,1%SDS,70%ethanol,30%iso-propanol,30%acetonitrile
forhigh-resolutionpreparativegelfiltrationchromatography,e.g,separatemAbsfromdimersandcontaminants
LegalInformation
HiLoadisaregisteredtrademarkofGEHealthcare
SuperdexisaregisteredtrademarkofGEHealthcare
Safety:
RIDADR
NONHforallmodesoftransport