Properties:
| RelatedCategories | Analytical/Chromatography,GEDesaltingColumns,GelFiltrationChromatography,HPLC,HPLC&FPLCColumnsforBiopolymerSeparations |
Description:
AnalysisNote
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FeaturesandBenefits
• Forrapidbufferexchange/desaltingofPCRproductsandotherDNAsinavolumeof10to100μlusingspin-columnchromatography.
• Excellentforrapidpurificationofnewlysynthesizedoligonucleotides>10-mersin100to150μlofdeprotectionsolutionusingspin-columnchromatography.
• Convenient:PrepackedwithSephadex®G-25DNAGradeandpre-equilibratedindistilledwatercontaining0.05%KathonCG/ICPBiocide.
• Readytouse:Requireslessthan4minfromsampleapplicationtocollectionofpurifiedproduct.
• Testedinnickase,singleanddouble-strandedexonucleaseandRNaseassays.
• Canalsobeusedfordesalting/bufferexchangeofDNAandremovalofunincorporatedrADIonucleotidesfromend-labeledoligonucleotides(atleast10basesinlength)inavolumeof10to100μl.
Generaldescription
MicroSpin™G-25columnsaredesignedfortherapidpurificationofDNAforuseinawiderangeofapplications,includingdesalting,bufferexchange,andremovalofunincorporatednucleotidesfromend-labelledoligonucleotides.
MicroSpin™G-25columnscanbeusedforanyDNAgreaterthan10basesinlengthandarethereforeanexcellentchoiceforthepurificationofoligonucleotidesorverysmallDNAfragmentsfollowingsynthesisoralabellingreaction.Theywillnotremoveordenatureenzyme.
LegalInformation
MicroSpinisatrademarkofGEHealthcare
SephadexisaregisteredtrademarkofGEHealthcare