Sigma-Aldrich/Ampicillin/59349-100MG/100MG

• CAS Number 69-53-4

• Empirical Formula (Hill Notation) C₁₆H₁₉N₃O₄S

• Molecular Weight 349.40

•  Beilstein Registry Number 1090925

•  EC Number 200-709-7

•  MDL number MFCD00005175

•  PubChem Substance ID 329758626

Properties:

Description:

Biochem/physiol Actions

Mode of Action: Ampicillin is a semisynthetic penicillin and a ?-lactam antibiotic that inhibits bacterial cell-wall synthesis by inactivating transpeptidases on the inner surface of the bacterial cell membrane.

Mode of Resistance: Administration with ?-lactamase cleaves the ?-lactam ring of Ampicillin and inactivates it.

Antimicrobial Spectrum: Effective against both Gram-positive (similar to benzylpenicillin) and Gram-negative bacteria (similar to tetracyclines and chloramphenicol.

Caution

This product has been reported stable as supplied at 25°C at 43% and 81% relative humidity for six weeks. Additional studies have shown that the stability of Ampicillin in solution is a function of pH, temperature and the identity of the buffer. It′s activity is quickly lost when stored above pH 7. Optimal storage conditions are suggested as 2-8°C, and pH 3.8-5 where its activity was retained at 90%+ for a week.

Packaging

This product is packaged in a bottomless glass bottle with the contents inside an inserted fused cone.

Preparation Note

Ampicillin is reported as slightly soluble in water, practically insoluble in alcohol, chloroform, ether and fixed oils but soluble in dilute acids or bases. The solution should not be autoclaved; a stock solution should be sterilized through filtration and stored frozen, where it will be stable for months.

Application

Refer to the product′s Certificate of Analysis for more information on a suitable instrument technique. Contact Technical Service for further support.

Safety:

Symbol 

  GHS07, GHS08