Properties:
| RelatedCategories | Analytical/Chromatography,HPLC,HPLCColumns,byBrandforSmallMolecules,HPLCColumns,byUSPCode,L67, |
| packaging | pkgof1 ea |
| application(s) | HPLC:suitable |
| L×I.D. | 25 cm×4.6 mm |
| matrixactivegroup | C18(octadecyl)phase |
| particlesize | 5 μm |
| poresize | 300 ? |
| suitABIlity | suitableforL67perUSP |
| modeofchromatography | Reversedphase |
Description:
Generaldescription
apHera™reversedcolumnsweredevolopedspecificallytoprovidethesuperioradvantagesofbothsilicaandpolystyrenecolumns,withoutthedisadvantagesofeither.Thiswasaccomplishedusingavinylalcoholcopolymerbasethatkeepsthesurfacewettedevenwithhighcarbonloads.Theporousstructurehasanaverageporediameterlargeenoughtoproduceidealresultsforsmallanalytes,peptidesandsmallproteins.ThesecolumnsequalsilicabasedcolumnsinseparationefficiencywithorganicsolventsbutprovideefficiencywithbufferedalkalinesolutionsnotpossIBLeonsilica.Oneofthemostsignificantfeaturesislogicialelutionorderofalkylatedbaseswhereretentionincreasesproportionatelywithincreasingchainlength.
FeaturesofapHera™ReversedPhaseColumns
• Stablevinylcopolymerbase
• StablepHrange2-12
• 300angstromporesize
• Stableinallorganicsolvents
• Expandedapplications:peptides,proteins
• Efficientseparationofanybasicsubstance
• Amenabilitytowashingandalkalinesolutions
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LegalInformation
apHeraisatrademarkofSigma-AldrichCo.LLC